A Priority Programme of the Deutsche Forschungsgemeinschaft

NANO-SELECT


  • ¹ Professor Dr. Volker Haucke
    Institut für Chemie und Biochemie
    Freie Universität Berlin
    Berlin
  • Dr. Andreas Jordan
    Centrum für Biomedizinische Nanotechnologie (CBN)
    Charité - Universitätsmedizin Berlin
    Berlin
  • Professor Dr. Eckart Rühl
    Institut für Chemie und Biochemie
    Abteilung Physikalische und Theoretische Chemie
    Freie Universität Berlin
    Berlin
  • Dr. Christina Graf
    Institut für Chemie und Biochemie
    Freie Universität Berlin
    Berlin
  • Dr. Annika Vogt
    Klinik für Dermatologie, Venerologie und Allergologie
    Campus Charité Mitte
    Charité - Universitätsmedizin Berlin
    Berlin
  • Professor Dr.-Ing. Jürgen Lademann
    Klinik fur Dermatologie, Venerologie und Allergologie
    Campus Charité Mitte
    Charité - Universitätsmedizin Berlin
    Berlin
  • ² Professor Dr. Achim Gruber
    Institut für Tierpathologie
    Fachbereich Veterinärmedizin
    Freie Universität Berlin
    Berlin
1 Phase 1: until Decembre 2010
2 Phase 2: since January 2011

Nanopartikels Containing Selective Probes: Interaction Mechanisms of Nanoparticles with Cell Membranes, Intracellular Uptake, and Transport

Nanoparticles of various size and shapes containing quantum dots or dyes as well as magnetic cores as selective probes are used to study their cell membrane penetration, uptake into cells, as well as transport processes. The particles are custom-made by colloidal chemistry and their properties are subsequently characterized by single-particle approaches. The particle interface is selectively functionalized, so that the uptake into different cellular systems is systematically investigated in a collaborative and interdisciplinary effort involving research groups from physical chemistry, membrane biochemistry, dermatology, and tumor research. Specifically, endocytosis into single cells is studied by cell biological and biochemical approaches. The penetration of nanoparticles through skin is studied as well as the accumulation into tumor cells. The particles are selectively detected via luminescence and X-ray microscopy and related optical and magnetic spectroscopic approaches. Finally, the particles are separated from their cellular environments in order to study the chemical changes induced by the cellular uptake and transport. The joint research effort aims to contribute significantly to the understanding of the mechanisms of nanoparticle uptake into living organisms, where cellular model systems, skin, and tumor cells are investigated.