Ein Schwerpunktprogramm der DFG

NPBIOMEM


  • Professor Dr. Christoph Bräuchle
    Department Chemie und Biochemie
    Ludwig-Maximilians-Universität München
    München
  • Professor Dr. Armin Reller
    Institut für Physik
    Universität Augsburg
    Augsburg
  • ¹ Privatdozent Dr. Stefan-Werner Schneider
    Klinik und Poliklinik für Hautkrankheiten
    Allgemeine Dermatologie und Venerologie
    Universitätsklinikum Münster
    Westfälische Wilhelms-Universität Münster
    Münster
  • Professor Dr. Achim Wixforth
    Lehrstuhl für Experimentalphysik I
    Universität Augsburg
    Augsburg
  • ¹ Dr. Matthias F. Schneider
    Lehrstuhl für Experimentalphysik I
    Universität Augsburg
    Augsburg
1 Phase 1: until Decembre 2010
2 Phase 2: since January 2011

Bioactivity and cellular uptake of distinct nanoparticles in human endothelial cells

Ubiquitary exposed to an increasing amount of nanoparticles (NPs) we are not able to escape NP uptake via our airways system, gastro-intestinal tract and skin. As a consequence NPs being able to penetrate these physiological barriers will enter our blood and lymphatic vessel system. The inner surface of the vessel system is covered by endothelial cells (EC) sensing all intravasculature physiological and artificial (such as NP) components. Endothelial cells play an essential role in controlling inflammation, coagulation, blood flow and blood pressure in human body. Therefore, any disturbance of endothelial cell activity may lead to inflammatory or coagulatory conditions with great impact on human health conditions. Due the multidisciplinary constellation of the team we designed an interdisciplinary approach to analyze the bioactivity of NP (designed by Prof. Reller (solid state chemistry)) on human endothelial cells (SW. Schneider (medicine)) using the combined strength of single NP tracing microscopy (Bräuchle (physical chemistry and cell biology)) and acoustically driven microfluidics (M.F. Schneider (biophysics)) and A. Wixforth (nanotechnology)). Mimicking the physiological conditions of our blood vessel by growing endothelial cells directly on a microfluidic lab on a chip, we will study the bioactivity and toxicity of NPs on human endothelial cells on short and long time scales (minutes to days). Here, we plan to utilize the optical acessibility and small size of our microfluidic reactor chip to fluorescently detect bioactivity, inflammatory response and toxicity of ECs by apoptotic markers, intracellular signalling and proinflammatory and coagulatory protein release. These studies will be compared with the uptake and pathway of single NPs in ECs by developing a hybrid between single NP tracking microscopy and a microfluidic reactor to control NP-cell collision rate and local NP concentration during the tracking process. These approaches allow to simultaneously measure the NP uptake and EC activity to finally correlate type and shape of NP, uptake mechanism and bioactivity.